snai1 protein  (Human Protein Atlas)

Journal: Cell Communication and Signaling : CCS

Article Title: The ester derivative Palmitoylcarnitine abrogates cervical cancer cell survival by enhancing lipotoxicity and mitochondrial dysfunction

doi: 10.1186/s12964-025-02218-8

Figure Lengend Snippet: List of primary and secondary antibodies

Article Snippet: SNAI1 , Rabbit , 1:3000 , Cell Signaling Technologies, USA , 3879 T.

Techniques:

Journal: Cell Communication and Signaling : CCS

Article Title: The ester derivative Palmitoylcarnitine abrogates cervical cancer cell survival by enhancing lipotoxicity and mitochondrial dysfunction

doi: 10.1186/s12964-025-02218-8

Figure Lengend Snippet: PC induces apoptosis, cell cycle arrest and reduces the expression of genes linked to cell proliferation and EMT. A Confocal images of AO/EtBr stained SiHa and HeLa cells before and after PC exposure. B Bar graph representing the cell cycle analysis of PC exposed and control SiHa and HeLa cells. C Representative Western blot images showing the reduction of genes associated with cell proliferation (AKT1 and ERK1/2) and cell cycle (Cyclin D1 and Cyclin E) in SiHa and HeLa treated with indicated concentrations of PC. D The bar graph represents the results of densitometric analysis of western blot images analyzed using ImageJ software. The data was normalized by comparing it with β-Actin. E Representative Western blot images showing the upregulation of CDH1 and downregulation of CDH2, VIM, c-Myc, SNAI1, and SLUG in SiHa and HeLa cells treated with PC. F Bar graph showing the quantitative analysis of CDH1, CDH2, VIM, c-Myc, SNAI1, and SLUG expression in PC-treated SiHa and HeLa cells. * P < 0.05, ** P < 0.01, and *** P < 0.001 indicates statistical significance

Article Snippet: SNAI1 , Rabbit , 1:3000 , Cell Signaling Technologies, USA , 3879 T.

Techniques: Expressing, Staining, Cell Cycle Assay, Control, Western Blot, Software

Journal: Journal of Cardiothoracic Surgery

Article Title: SNAI1: a key modulator of survival in lung squamous cell carcinoma and its association with metastasis

doi: 10.1186/s13019-024-03044-8

Figure Lengend Snippet: Expression of SNAI1 in tumor tissues and compared to normal control tissues. A . Tumor tissues and compared to normal control tissues. B . Different stages. C . Metastasis status

Article Snippet: The subcellular localization of the SNAI1 protein within LUSC tissue cells was investigated using immunohistochemistry data sourced from the Human Protein Atlas database.

Techniques: Expressing, Control

Journal: Journal of Cardiothoracic Surgery

Article Title: SNAI1: a key modulator of survival in lung squamous cell carcinoma and its association with metastasis

doi: 10.1186/s13019-024-03044-8

Figure Lengend Snippet: Optimal Threshold Analysis of SNAI1 expression. Optimal threshold analysis software(X-tile Software) was employed to stratify SNAI1 expression into three-tier ( A , B ) and two-tier ( C , D ) classifications for patient prognosis analysis. Impact of Metastasis status ( E ) and SNAI1 Expression ( F ) on LUSC Prognosis

Article Snippet: The subcellular localization of the SNAI1 protein within LUSC tissue cells was investigated using immunohistochemistry data sourced from the Human Protein Atlas database.

Techniques: Expressing, Software

Journal: Journal of Cardiothoracic Surgery

Article Title: SNAI1: a key modulator of survival in lung squamous cell carcinoma and its association with metastasis

doi: 10.1186/s13019-024-03044-8

Figure Lengend Snippet: Validation of SNAI1 expression and prognosis correlation in multiple LUSC datasets (Gene Expression Omnibus (GEO) database: GSE3141, GSE29013, GSE4573, GSE157011)

Article Snippet: The subcellular localization of the SNAI1 protein within LUSC tissue cells was investigated using immunohistochemistry data sourced from the Human Protein Atlas database.

Techniques: Expressing

Journal: Journal of Cardiothoracic Surgery

Article Title: SNAI1: a key modulator of survival in lung squamous cell carcinoma and its association with metastasis

doi: 10.1186/s13019-024-03044-8

Figure Lengend Snippet: Clinical characteristics

Article Snippet: The subcellular localization of the SNAI1 protein within LUSC tissue cells was investigated using immunohistochemistry data sourced from the Human Protein Atlas database.

Techniques:

Journal: Journal of Cardiothoracic Surgery

Article Title: SNAI1: a key modulator of survival in lung squamous cell carcinoma and its association with metastasis

doi: 10.1186/s13019-024-03044-8

Figure Lengend Snippet: Univariate and multivariate Cox regression analyses

Article Snippet: The subcellular localization of the SNAI1 protein within LUSC tissue cells was investigated using immunohistochemistry data sourced from the Human Protein Atlas database.

Techniques:

Journal: Journal of Cardiothoracic Surgery

Article Title: SNAI1: a key modulator of survival in lung squamous cell carcinoma and its association with metastasis

doi: 10.1186/s13019-024-03044-8

Figure Lengend Snippet: The expression of SNAI1 protein in LUSC associated with prognosis

Article Snippet: The subcellular localization of the SNAI1 protein within LUSC tissue cells was investigated using immunohistochemistry data sourced from the Human Protein Atlas database.

Techniques: Expressing

Journal: Journal of Cardiothoracic Surgery

Article Title: SNAI1: a key modulator of survival in lung squamous cell carcinoma and its association with metastasis

doi: 10.1186/s13019-024-03044-8

Figure Lengend Snippet: The hallmark gene sets correlated with SNAI1 expression

Article Snippet: The subcellular localization of the SNAI1 protein within LUSC tissue cells was investigated using immunohistochemistry data sourced from the Human Protein Atlas database.

Techniques:

Journal: Journal of Cardiothoracic Surgery

Article Title: SNAI1: a key modulator of survival in lung squamous cell carcinoma and its association with metastasis

doi: 10.1186/s13019-024-03044-8

Figure Lengend Snippet: SNAI1 Expression Correlates with Immune Checkpoint Molecules in LUSC

Article Snippet: The subcellular localization of the SNAI1 protein within LUSC tissue cells was investigated using immunohistochemistry data sourced from the Human Protein Atlas database.

Techniques: Expressing

Journal: Journal of Cardiothoracic Surgery

Article Title: SNAI1: a key modulator of survival in lung squamous cell carcinoma and its association with metastasis

doi: 10.1186/s13019-024-03044-8

Figure Lengend Snippet: Identification of potential molecular targeted drugs for SNAI1

Article Snippet: The subcellular localization of the SNAI1 protein within LUSC tissue cells was investigated using immunohistochemistry data sourced from the Human Protein Atlas database.

Techniques:

Journal: Cancer Reports

Article Title: Knockdown of PRDX2 Inhibits the Proliferation, Growth, Migration, Invasion, and MMP9 Activity of Ewing's Sarcoma Cells Cultured In Vitro

doi: 10.1002/cnr2.2122

Figure Lengend Snippet: Knockdown of PRDX2 inhibits activation of AKT signaling pathway, and SNAI1 is involved in activating PRDX2 transcription. (A–C) The levels of AKT, phosphorylated AKT, and a downstream target protein cyclin D1 of the AKT signaling pathway detected by western blot. (D) The predicted binding site of SNAI1 on PRDX2 gene promoter. Data from UCSC. (E) Dual‐luciferase experiment demonstrated that SNAI1 activated the transcription of downstream gene by binding to predicted binding site. (F) Chromatin co‐immunoprecipitation experiment revealed the recruitment and enrichment of SNAI1 to the PRDX2 gene promoter. * p < 0.05.

Article Snippet: DNA cross‐linked to SNAI1 protein was precipitated with SNAI1 antibody (13099‐1‐AP, Proteintech) or non‐specific IgG (1:200, Sigma).

Techniques: Knockdown, Activation Assay, Western Blot, Binding Assay, Luciferase, Immunoprecipitation

Journal: Cancer Research Communications

Article Title: MARCH2, a Novel Oncogene-regulated SNAIL E3 Ligase, Suppresses Triple-negative Breast Cancer Metastases

doi: 10.1158/2767-9764.CRC-23-0090

Figure Lengend Snippet: MARCH2 promotes ubiquitination and downregulation of SNAIL. A, HA-tagged WT, but not RING domain–mutant (W97A), MARCH2 downregulates SNAIL levels in MDA-MB-231 cells. B, MARCH2 coprecipitates with SNAIL in MDA-MB-231 cells coexpressing FLAG-SNAIL and HA-MARCH2. C, WT, but not RING domain– or transmembrane domain–mutant, MARCH2 ubiquitinates SNAIL. FLAG-Snail was immunoprecipitated from MDA-MB-231 cells coexpressing FLAG-Snail, HA-Ubiquitin and GST-tagged MARCH2 constructs. Immunoprecipitates were blotted with antibodies to HA to assess ubiquitination. D, MARCH2 ubiquitinates SNAIL in in vitro ubiquitination assay. Reactions were performed with recombinant SNAIL (Myc-tagged) in the presence of ubiquitin, recombinant E1 (UBE1), recombinant E2 (UBE2D3) and/or recombinant MARCH2.

Article Snippet: The in vitro ubiquitination reactions were performed using 2 µmol/L recombinant human SNAIL protein (Origene TP304581), 3 µmol/L recombinant human March2 E3 ligase (Origene TP307517), 100 µmol/L Ubiquitin (R&D Systems U-100H-10M), 100 nmol/L recombinant E1(UBE1, Biotechne E-305), 500 nmol/L recombinant E2 (UBE2D3, Biotechne E2-627), 1X ATP Energy Regeneration buffer (Enzo BML-EW9810-0100) in a 25 µL reaction buffer containing 50 mmol/L Hepes, pH 8.0, 50 mmol/L NaCl, and 1 mmol/L TCEP.

Techniques: Mutagenesis, Immunoprecipitation, Construct, In Vitro, Ubiquitin Assay, Recombinant

Journal: International journal of molecular medicine

Article Title: Knockdown of KLK12 inhibits viability and induces apoptosis in human colorectal cancer HT-29 cell line.

doi: 10.3892/ijmm.2019.4327

Figure Lengend Snippet: Figure 6. Expression of epithelial‑mesenchymal transition‑associated and apoptosis‑associated proteins is altered in HT‑29 cells treated with siKLK12 or KLK12 overexpression plasmids. (A) The mRNA levels of E‑cadherin, vimentin, Snail, MMP‑2 and MMP‑9 were analyzed by reverse transcription quantita- tive polymerase chain reaction. (B and C) The protein levels of E‑cadherin, vimentin, Snail, MMP‑2 and MMP‑9 were analyzed by western blot analysis. (D and E) The protein levels of Bcl‑2, Bax protein and cleaved caspase‑3 were analyzed by western blot analysis. *P<0.05 and **P<0.01 vs. NC group. ^P<0.05 and ^^P<0.01 vs. mock group. The ‘KLK12’ group represents the KLK12 overexpression group. KLK12, kallikrein‑related peptidase 12; si, small interfering RNA; E‑cadherin, epithelial cadherin; Snail, zinc finger protein SNAI1; MMP, matrix metalloproteinase; NC, negative control.

Article Snippet: The antibodies used in the study were as follows: Anti-GAPdH (mouse; 1:1,000; cat. no. LS-B1625; LifeSpan BioSciences, Inc.); anti-epithelial (E)-cadherin (mouse; 1:1,000; cat. no. ab1416; Abcam); anti-vimentin (rabbit; 1:1,000; cat. no. ab92547; Abcam); anti-matrix metalloproteinase (MMP)-2 (rabbit; 1:1,000; cat. no. ab37150; Abcam); anti-MMP-9 (rabbit; 1:1,000; cat. no. ab73734; Abcam); anti‐zinc finger protein SNAI1 (Snail; rabbit; 1:1,000; cat. no. 3879; cell Signaling Technology, Inc.); anti-cleaved caspase-3 (rabbit; 1:1,000; cat. no. ab13847; Abcam); anti-Bax (rabbit; 1:1,000; cat. no. ab32503; Abcam); anti-Bcl-2 (rabbit; 1:1,000; cat. no. ab32124; Abcam); anti-AMPK (mouse; 1:1,000; cat. no. ab32047; Abcam); anti-phosphorylated (p)-AMPK (rabbit; 1:1,000; cat. no. ab133448; Abcam); anti-mTOR (rabbit; 1:1,000; cat. no. ab32028; Abcam); and anti-p-mTOR (rabbit; 1:1,000; cat. no. ab84400; Abcam).

Techniques: Expressing, Over Expression, Reverse Transcription, Polymerase Chain Reaction, Western Blot, Small Interfering RNA, Negative Control